If you do not have the adobe acrobat reader necessary for viewing this documentation, download your free acrobat reader now. Current standardofcare treatment for malignant cancers includes radiotherapy and adjuvant chemotherapy. To speak with a representative, please fill out the contact form below or call us directly. Checkout sample for testing the performance of the agilent 3100 offgel fractionator. The seven individual proteins were loaded on cm ipg strips ph 3 to 10 by rehydration for 1 hour at ambient temperature, using the tray. The agilent technologies 33220a is a 20 mhz synthesized function generator with builtin arbitrary waveform and pulse capabilities. Database contains 3 agilent technologies 6490 manuals available for free online viewing or downloading in pdf. All agilent documentation is compiled in adobe portable document format pdf. U1253a user s and service guide ix in this guide d e t r a t s g n i t t e1g this chapter contains information on the u1253a true rms oled multimeter front panel, rotary switch, keypad, display, terminals and rear panel.
Results are highly reproducible, and the agilent 3100 offgel fractionator is simple and easy to use. Related manuals for agilent technologies 3100 offgel fractionator laboratory equipment agilent technologies varian 3800 gc operating instructions manual 6 pages laboratory equipment agilent. The agilent technologies 3100 offgel fractionator with control module uses a novel isoelectric focusing technique to achieve excellent pibased fractionation. View online or download agilent zorbax 300sbc18 user manual. To get the most out of the products we recommend that you read the accompanying product user manual, this programmers guide and. Power consumption ambient operating temperature humidity operating altitude safety standards. The agilent 3100 offgel fractionator uses a novel isoelectric focusing technique to achieve excellent pibased fractionation with high reproducibility. Agilent technologies 3100 offgel fractionator pdf user manuals. The information contained in this document is subject to change without notice.
Agilent 3100 offgel fractionator user manual appendix noncondensing conditions ordering information this chapter contains details on noncondensing conditions as well as ordering information for consumables and spare parts. Two methods were tested for offgel fractionation of. Development of an inhouse protocol for the offgel fractionation of. Enter the desired item number and quantity to obtain product availability and pricing.
Achieve userindependent results with minimal manual intervention and excellent. Samples can be fractionated into 12 or 24 fractions for standard obtain greater reproducibility fractionation by offgel. The resulting fractions are in solution, making recovery. This application note illustrates the effectiveness of offgel electrophoresis with the agilent 3100 offgel fractionator. Stateoftheart user interface for online control of each individual sample. View and download agilent technologies 3100 offgel fractionator user manual online. The agilent handheld control module selfconfigures all modules for total system control using controllerarea network can communication and cabling. Agilent 3100 offgel fractionator donald danforth plant. This way youll save time on finding the necessary info.
We use cookies on this site to enhance your user experience. Companies, names, and data used in examples herein are fictitious unless otherwise noted. This advanced fractionator features a userfriendly. The combination of fractionation, intermediate controls, and accurate mass determination at high resolution makes this solution a valuable tool for characterization and quality control qc analysis of mabs for the biopharmaceutical research and development. Congratulations on having purchased an agilent technologies acqiris data conversion product. Featuring agilent available for buying right now on the internet. User manuals, guides and specifications for your agilent technologies g1290 laboratory equipment. Download agilent technologies 3100 offgel fractionator. Agilent 6495 triple quadrupole lcms with 1260 front end. Laboratory equipment agilent technologies 3100 offgel fractionator user. Agilent g3100a offgel fractionator for sale online ebay. User manuals, agilent technologies laboratory equipment operating guides and service manuals. Citeseerx document details isaac councill, lee giles, pradeep teregowda. Agilent technologies 3100 offgel fractionator user manual pdf.
View online or download agilent zorbax 300sbc8 user manual. Database contains 1 agilent technologies g4204a manuals available for free online viewing or downloading in pdf. Database contains 1 agilent technologies g1290 manuals available for free online viewing or downloading in pdf. Protein fractionation achieve highest resolution the agilent 3100 offgel fractionator provides plbased fractionation of peptides or proteins with exceptional resolution. Chapter 1 getting started chapter 1 pro vides unpac king, initial insp ection, and preparation. User manuals, guides and specifications for your agilent technologies g4204a laboratory equipment. Agilent gcmsd 5977 agilent technologies life sciences. Insolution fraction recovery for easy transfer to lcms. With its new stateoftheart webbased user interface offgel electrophoresis has just become even more intuitive to use.
Agilent 34401a digital multimeter with two test leads one red, one black agilent 33220a functionarbitrary waveform generator introduction. Varian 3800 gc laboratory equipment pdf manual download. Agilent 3100 offgel fractionator dramatically improve your protein and peptide identification the easytouse agilent 3100 offgel fractionator uses novel isoelectric focusing to achieve highly reproducible pibased fractionation. Agilent 33250a at a glance the agilent technologies 33250a is a highperformance 80 mhz.
The agilent 3100 offgel fractionator provides robust, repeatable insolution fraction recovery that ensures lcms assays are optimized for the maximum number of proteins. Application note the comprehensive analysis of complex protein or peptide mixtures by lcms can be improved by including a prefractionation step in the workflow. Protein isoelectric focusing with the agilent 3100 offgel fractionator. See figure 4 in section 1 of the agilent 3100 user manual. Inhibition of mnk pathways enhances cancer cell response. View online or download agilent technologies 3100 offgel fractionator user manual. Although a few comparisons of the relative separation efficiencies of 2. Related manuals for agilent technologies 3100 offgel fractionator laboratory equipment agilent technologies varian 3800 gc operating instructions manual 6 pages laboratory equipment agilent technologies varian 325ms lcms preinstallation instructions 14 pages. Agilent 86140b series optical spectrum analyzer users guide. Samples can be fractionated into 12 or 24 fractions for standard or highest resolution. Electrophoresis system for pibased fractionation of proteins or peptides ingel or in offgel mode. This website uses cookies to improve user experience. For pibased peptide separation, the 3100 offgel fractionator and the offgel kit ph 310 both agilent technologies with a 24well setup. An introduction to using the agilent 54622d digital.
Conventional isoelectric focusing with the agilent 3100. This guide describes the proper use of the reagent kits with the agilent 3100 offgel fractionator. Results are highly reproducible, and the agilent 3100 offgel fractionator. Today, for almost any proteomic analysis, robust and reproducible fractionation. Fractionator in the offgel mode and the agilent 3100 offgel high. Integration of the offgel system is easy and ultimately increases sensitivity of subsequent ms detection. View and download agilent technologies varian 3800 gc operating instructions manual online. Optimization of itraq labelling coupled to offgel fractionation as. Efficient protein fractionation and identification with. Agilent 3100 offgel fractionator fractionator used. Agilent 3100 offgel fractionator arc scientific used lab. Efficient fractionation and improved protein identification by peptide. Agilent 3100 offgel fractionator agilent 6530 accuratemassquadrupole timeofflight.
Agilent technologies g23b for agilent technologies 3100. Agilent 3100 offgel fractionator user manual agilent technologies 1 product overview intended use 14 features 16 instrument and accessories 17 this chapter gives an overview of the agilent 3100 offgel fractionator. Stateoftheart user interface for intuitive instrument setup and control and online. Analysis of a protein ief marker mix with the agilent 2100 bioanalyzer before and after offgel electrophoresis 24 fractions, ph 310. Agilent technologies g4204a manuals and user guides. Download 254 agilent technologies laboratory equipment pdf manuals. This advanced fractionator features a user friendly interface and can connect to a pc or be operated from a smart phone when connected to a. The agilent technologies g23b is a handheld control module for use in the 3100 series offgel fractionator to provide complete local control and monitoring. Tous les catalogues et fiches techniques pdf agilent.
User manuals, guides and specifications for your agilent technologies varian 3800 ms laboratory equipment. Agilent technologies laboratory equipment user manuals. View online or download 1 manuals for agilent technologies g1290. The checkout sample contains a single protein which will focus into only one fraction when used with the low resolution kit, ph 310 order no. Citeseerx the agilent 3100 offgel fractionator authors. Agilent 2100 bioanalyzer and agilent 2200 tapestation systems agilent 3100 offgel fractionator systems, consumables and supplies. Agilent 6495 triple quadrupole lcms with 1260 front end in arcade, ny, usa. This address reflects the default ip address of the agilent 3100 offgel fractionator. User s guide software overview guide performance verification manual online information system in this book. Offgel fractionation, recently developed by agilent technologies, provides. Each hplc module is supplied with a reference manual which describes installation, troubleshooting, repair, parts and theory of operation. Currents of each sample are measured and displayed online individually. Includes handheld controller, preinstalled software, manua.
Sample complexity and large dynamic range of protein and peptide concentrations are major hurdles in the analysis of low abundance proteins. Agilent 3100 offgel fractionator instrument biosurplus. It describes the intended use and the fracti onation principles of the instrument. The agilent 3100 offgel fractionators uses a novel isoelectric focusing technique to achieve. View online or download 3 manuals for agilent technologies 6490. Tutti i cataloghi e le schede tecniche agilent technologies. Benefits of the agilent 3100 offgel fractionator pibased offgel fractionation. Agilent 3100 offgel fractionator peptide protein electrophoresis pendant g3100a. Agilent 3100 offgel fractionator peptide protein electrophoresis.
The resulting fractions are in solution, making recovery for lcms analysis much easier than with oldfashioned gels. Efficient peptide fractionation and improved protein. This manual contains information for troubleshooting and repairing to the component level the agilent model 66332a dynamic measurement dc source and the agilent model 6632b, 6633b, 6634b system dc power supplies. Manual this man ual, the op eration man ual for the hp 4284a precision lcr meter, con tains ten c hapters plus app endixes, organized for the con v enience of the rst time user. Installation manual, quick start manual, manual book.
Peptide fractionation is extremely important for the comprehensive analysis of complex protein mixtures. Conventional isoelectric focusing with the agilent 3100 offgel fractionator introduction this technical overview demonstrates the ability of the agilent 3100 offgel fractionator to fractionate proteins or peptides based on their isoelectric points pi in liquid phase offgel mode. Agilent 3100 offgel fractionator the lab world group. Benefits of the agilent 3100 offgel fractionator pibased offgel fractionation with liquidphase recovery for easy transfer to downstream techniques. High resolution liquid chromatograph mass spectrometer. Besides, its possible to examine each page of the guide singly by using the scroll bar. Agilent technologies g1290 manuals and user guides. Offgel electrophoresis with the agilent 3100 offgel fractionator provides protein and peptide separation with excellent resolution and easy, insolution recovery. Amicon ultra, millipore according to the manufacturer instructions. Conventional isoelectric focusing with the agilent 3100 offgel. The agilent 3100 offgel fractionator uses a novel isoelectric focusing technique to achieve excellent pibased fractionation. Agilent 3100 offgel fractionator instrument specifications type weight dimensions height.
Agilent 3100 offgel fractionator arc scientific used. This system makes recovery for lcms analysis easier than traditional gels as the resulting fractions are in solution. User manuals, guides and specifications for your agilent technologies 6490 analytical instruments, laboratory equipment. Agilent technologies 3100 offgel fractionator with control module. Evaluation of peptide fractionation strategies used in. The agilent 3100 offgel fractionator provides pibased fractionation of peptides or proteins with exceptional resolution. Offgel user manual 67 4 68 operation performing a fractionation offgel user manual agilent 3100 offgel fractionator user manual 5 maintenance cleaning the 3100 offgel fractionator 70 firmware update 76 this chapter describes cleaning procedures that should be used to do maintenance on your instrument at regular intervals or before every use.
Agilent 53220a53230a 350 mhz universal frequency counter timer. Results are highly reproducible, and the agilent 3100 offgel fractionator is now even easier to use with its new webbased user interface. Agilent 531a2a181a 225 mhz counter agilent 531a2a181a assemblylevel service guide this guide describes how to service the agilent 531a and agilent 532a universal counters, and the agilent 53181a frequency counter. Its combination of benchtop and system features makes this function. After y ou receiv ey our hp 4284a, b egin with chapter 1. Agilent technologies 3100 offgel fractionator manuals. Individual sample diagnostics for a quick and easy check of fractionation quality with its stateoftheart user interface the 3100 offgel fractionator measures and records voltage, power and current during fractionation in offgel and traditional ief mode.
Efficient protein fractionation and identification with the agilent 3100 offgel fractionator abstract the sample fractionation steps done prior to mass detection are critically important for the comprehensive analysis of complex protein mixtures. Agilent technologies varian 3800 gc operating instructions manual. According to the manual, the fractionator can be controlled via the control. Manufacturers product overview the agilent 3100 offgel fractionator provides. This is a basic introduction to four pieces of equipment listed above. The agilent 3100 offgel fractionator system fractionates proteins or peptides based on their isoelectric points pi in liquid phase offgel mode. Hereafter all models will be referred to as the dc power supply. Key instrumentation eastern virginia medical school evms. Agilent technologies varian 3800 ms manuals and user. Agilent hewlett packard 4263a, meter as photo, sn4352, for part not working d. To look into this further, peptide amino acid structures were manually drawn. Agilent 3100 offgel plbased protein peptide electrophoresis fractionator.